Determination of Antioxidant Activity of Leave Extracts of Albizia chevalieri Using Free Radical Scavenging Activity Assay

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Alhassan M. Garba
Habiba R. Isa
Sadiq Abubakar
Saudat Ja’afar


Dried and powdered leaves of Albizia chevalieri were extracted using ethanol. The extract was fractionated to give methanol, chloroform and pet-ether. The four extracts obtained; ethanol, chloroform, methanol and pet-ether were evaluated for antioxidant activity using 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) free radical scavenging activity assay. The results of the DPPH scavenging activity indicated a concentration-dependent antioxidant activity. The DPPH scavenging activity of the ethanol, chloroform and methanol extracts were found to be promising. There is no significant difference in the antioxidant activity between the ethanol, chloroform and methanol extracts with that of standard Ascorbic acid at 10, 25, 250 and 500 μg/ml concentrations. This showed that the ethanol, chloroform and methanol leave extracts of the plant has the potency of scavenging free radicals in vitro and may provide leads in the ongoing search for natural antioxidants from Nigerian medicinal plants to be used in treating diseases related to free radical reactions.

Albizia chevalier, antioxidant, free radical, extract, scavenging activity assay, DPPH.

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How to Cite
Garba, A., Isa, H., Abubakar, S., & Ja’afar, S. (2019). Determination of Antioxidant Activity of Leave Extracts of Albizia chevalieri Using Free Radical Scavenging Activity Assay. International Research Journal of Pure and Applied Chemistry, 19(1), 1-6.
Original Research Article


Burkill HM. The useful plant of west tropical Africa. Families J–L. Royal Botanical Gardens, Kew. 1995;3:207– 208.

Aliyu AB, Musa AM, Ibrahim MA, Ibrahim H, Oyewale AO. Preliminary phytochemical screening and antioxidant activity of leave extract of Albizia chevalieri harms. Bayero Journal of Pure and Applied Sciences. 2009;1(2):149-153.

Muchuweti M, Nyamukonda L, Chagonda, LS, Ndhlala AR, Mupure C, Behura M. Total phenolic content and antioxidant activity in selected medicinal plants of Zimbabwe. Int. J. Food Sci. Tech. 2006;41: 33–38.

Tempone AG, Sartorelli P, Teixeira D, Prado FO, Calixto IARL, Lorenzi H, Melhem M. Brazilian flora extract as source of nivel anileishmanial and antifungal compound. Mem, 1st Oswaldo Cruz. 2008;103(5):443–449.

Freiburghaus F, Owgwal EN, Nkuny MH, Kaminsky R, Brun R. In vitro anti-trypanosomal activity of African plants used in traditional medicine in Uganda to treat sleeping sickness. Trop. Med, Int. Health. 2007;1(6):765–771.

Agyare C, Kofuer GA, Mensah AY, Agyemang DO. Boletin Latinoamericano Y. del Cribe de plantas Medicinales. Y Aromatica. 2006;5(2):31–35.

Kohlera I, Jenett–Siema K, Siemsb K, Herna ndezc MA, Ibarrac RA, Berendsohnd WG, Bienzlee U, Eicha E. In vitro antiplasmodial investigation of medicinal plants from El Salvadorz. Naturforsch. 2002;57c:277–281.

Rashid RB, Chowdhury R, Jabbar A, Hassan GM, Rashid MA. Constituents of Albizia lebbeck and antibacterial activity of isolated flavones derivatives. Saudi Pharm. J. 2003;11(1-2):52–56.

Karou D, Ndaembega WMC, Outtara L, Ilboudo DP, Canini A, Nikiema JP, Simpore J, Collizi V, Traore AS. African ethnopharmacology and new drug discovery. Medicinal and Aromatic plant Science and Biotechnology. 2006;1:1–9.

Kedare Sagar B, Singh RP. Genesis and development of DPPH method of anti-oxidant assay. J Food Sci Technol. 2011; 48(4):412-422.

Tiwari A. Imbalance in antioxidant defense and human diseases: multiple approach of natural antioxidants therapy. Curr. Sci. 2001;81:1179-1187.

Braugghler JM, Duncan CA, Chase LR. The involvement of Iron in lipid peroxidation: Importance of ferrous to ferric ratio in initiation. J. Biol. Chem. 1986;61: 102-182.

Perez–Jimenez J, Saura–Calixto F. Anti-oxidant capacity of dietry polypenols determined by ABTS assay: A kinetic expression of the results”. International Journal of food science and Technology. 2008;43:185-191.

Perez–Jimenez J, Arranz S, Tabernero M, Diaz-Rubia ME, Serrano J, Goni I, Saura-Calixto F. Updated methodology to determine antioxidant capacity in plant foods, oils and beverages: Extraction measurement and expression of results). Food Research International. 2008;41(3): 274–285.

Prakash A. Antioxidant activity medallion laboratories analytical progress. 2001; 19(2).

Fatope MO, Ibrahim H, Takeda Y. Screening of higher plants reputed as pesticides using brine shrimp lethality assay. Int. J. Pharmacog. 1993;31:250- 56.

Bozin B, Mimica-Dukic N, Samojlik I, Goran A, Igic R. Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae). Food Chem. 2008;111:925-929.

Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 2005;53:1841- 1856.

Karagozler AA, Erdag B, Emek YC, Uygum DA. Antioxidant activity and proline content of leaf extracts from Dorystoechas hastate. Food Chem. 2008;111:400-407.

Mensor LI, Menezes FS, Leitao GG, Reis AS, dos Santos T, Coube CS, Leitao SG. Screening of Brazilian plants extracts for antioxidant activity by the use of DPPH free radical method. Phytother. Res. Tech. 2001;15:127–130.