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Silica gel (70-230 mesh ASTM) was packed into a column (45 cm × 3 cm) using the dry method. About 7 g of extract was mixed with 30 g of silica gel and allowed to dry. It was then loaded onto the column and successively eluted initially with pet ether 100%, followed by 10%, 20%, 40%, 60%, 80% chloroform in pet-ether; followed by 100% chloroform; 20%, 50% ethyl acetate in chloroform; followed by 100% ethyl acetate and then 20% and 50% methanol in ethyl acetate. 25 fractions were collected in 50 ml aliquots and bulked together according to their TLC profiles and Rf. The bulked fraction was further column chromatographed over silica gel (70-230 mesh) using a pipette and isocratically eluting with pet-ether: chloroform: methanol 67:25:8. 30 fractions of 2 ml each were collected. Compound C-3 (200 mg) was obtained from the fractions 1-15 as an off-white amorphous powder. The combination of IR, 1HNMR, 13CNMR, gCOSY, HMBC, HSQC and Mass spectral data on this off-white powder has led to an unambiguous assignment and the compound, an amide, isolated from the bioactive fraction of F. exasperata has the chemical name (2S)2-hydroxy-N-((3R,4R)-1,3,4-trihydroxytridecan-2-yl)undecamide. The acclaimed medicinal uses of this plant such as antihypertensive, anti-inflammatory, anti-arthritic, anti-ulcerogenic, anti-microbial and anti-oxidant, among others made it attractive to the authors.
UNESCO. Culture and health, orientation texts – world decade for cultural development 1988-1997. Document CLT/DEC/PRO – 1996, Paris, France. 1996;129.
UNESCO. FIT/504-RAF-48 Terminal Report: Promotion of ethnobotany and the sustainable use of plant resources in Africa. Paris. 1998;60.
Hamburger M, Hostettmann K. Bioactivity in plants: The link between phytochemistry and medicine. Phytochemistry. 1991;30(12): 3864–3874.
Taylor JLS, Rabe T, McGaw LJ, Jager AK, van Staden J. Towards the scientific validation of traditional medicinal plants. Plant Growth Regulation. 2001;34:23- 37.
Gurib-Fakim A. Medicinal plants: Traditions of yesterday and drugs of tomorrow. Molecular Aspects of Medicine. 2006;27:1-93.
Bagnis CI, Deray G, Baumelou A, Le Quintrec M, Vangerweghem JL. Herbs and the kidney. AJKD Am. J. Kidney Dis. 2004;44:1-11.
Gill GV, Redmond S, Garrat F, Paisey R. Diabetes and alternative medicine; cause for concern. Diabetic Medicine. 1994;11: 210-213.
Gester WM. Therapeutic landscapes: Medicinal issues in light of the new cultural geography. Social Science and Medicine. 1992;34:735-746.
WHO. WHO traditional medicine strategy 2002-2005. Geneva. 2002;1-62.
Sirisha N, Sreenivasulu M, Sangeeta K, Chetty CM. Antioxidant properties of Ficus species: A review. International Journal of PharmTech Research. 2010;2(4):2174-2182.
Irvin FR. Woody plants of Ghana. Oxford University Press, Amen House London. 1961;431.
Berg CC. Ficus exasperata Vahl (Family Moraceae). Flora Zambesiaca. 1991;9(6): 13.