A Profoundly Touchy Approved Spectrofluorimetric Approach for Assurance of Ixabepilone as Anticancer Drug by Utilizing Its Quenching Impact on Acetoxymercuric Fluorescein Reagent
International Research Journal of Pure and Applied Chemistry,
A dependable, sensitive, basic and cheap spectrofluorimetric approach has been created for test of sulfur-containing drug; ixabepilone in bulk powder, vials and human plasma. The approach depends on the quenching effect of ixabepilone on the fluorescence intensity of acetoxymercuric fluorescene (AMF) reagent at λem of 530 nm and λex of 500 nm. Parameters which will control the reaction such as pH, AMF solution concentration, temperature, time and solvents were examined and optimized. According to the optimized conditions, the proposed approach was practiced over the concentration area of 20-100 ng mL-1 with adequate linearity (r = 0.9998). The developed approach was approved confirming to ICH rules in terms of accuracy, precision, linearity, LOD and LOQ. The proposed approach was practiced to analyze ixabepilone in Ixempra® vials with satisfactory recovery % of 99.89 and RSE% of 1.24. The results achieved were compared to those achieved by an already reported HPLC approach.
- ixabepilone as anticancer
- acetoxymercuric fluorescein reagent
- human serum.
How to Cite
S, G. Novel cytotoxic agents: epothilones. Am J Health Syst Pharm. 2008;65:10 Suppl 3.
DOI:10.2146/ajhp080089. PMID 18463327
Lee FY, Borzilleri R, Fairchild CR, Kamath A, Smykla R, Kramer R, Vite G. Preclinical discovery of ixabepilone, a highly active antineoplastic agent. Cancer Chemother. Pharmacol. 2008;63(1):157–66.
DOI: 10.1007/s00280-008-0724-8. PMID 18347795
Bareaton LL, Hilal-Dandan R, Knollmann BC. Goodman, & Gilman's the pharmacological basis of therapeutics. McGraw-Hill Education New York; 2018.
Vulfovich M, Rocha-Lima Cea. Novel advances in pancreatic cancer treatment. Expert Rev Anticancer Ther. 2008;8(6):993–1002.
DOI: 10.1586/14737188.8.131.523. PMID 18533808. S2CID 20049942
Zeng J, Mylott W, Arnold M, Waltrip J, Lacono L, Mariannino T, Stouffer B. Liquid chromatography and tandem mass spectrometry for the quantitative determination of ixabepilone (BMS-247550, Ixempra™) in human plasma: Method validation, overcoming curve splitting issues and eliminating chromatographic interferences from degradants. Journal of Chromatography B. 2010;878(5-6):525-537.
Beumer JH, Garner RC, Cohen MB, Galbraith S, Duncan GF, Griffin T, Beijnen JH, Schellens JHM. Human mass balance study of the novel anticancer agent ixabepilone using accelerator mass spectrometry. Investigational new drugs. 2007;25(4):327-334.
Çömezoğlu SN, LY Van T, Zhang D, Humphreys WG, Bonacorsi SJ, Everett DW, Cohen MB, Gan J, Beumer JH, Beijnen JH. Biotransformation profiling of [14C] ixabepilone in human plasma, urine and feces samples using accelerator mass spectrometry (AMS). Drug metabolism and pharmacokinetics. 2009;24(6):511-522.
Colovos G, Haro M, Frewer H. Reactions of 2-, 7—Bis (Acetoxymercuri)-fluorescein with certain complexing anions . Talanta. 1970;17:273-278.
Axelrod HD, Cary HJ, Bonelli JE, Lodge JP. Fluorescence determinationof sub-parts per billion hydrogen sulfide in the atmosphere, Anal. Chem. 1969;41(13):1856-1858.
Jayaraman S, Walia R, Alagirisamy N. Fluorescein mercuric acetate – a novel sensor for oral malodour detection. Sens. Actuators, B. 2010;148(1):54-58.
Toda K, Ohira SI, Ikeda M. Micro-gas analysis system gas comprising a microchannel scrubber and a micro fluorescence detector for measurement of hydrogen sulfide. Anal. Chim Acta. 2004;511(1):3-10.
Haggag RS, Gawad DA, Belal SF, Elbardisy HM. Spectrophotometric and spectrofluorimetric determination of mesna, acetylcysteine and timonacetic acid through the reaction with acetoxymercuri fluorescein. Anal. Methods. 2016;8(11):2479-2493.
Shaalan R. Improved spectrofluorimetric methods for determination of penicillamine in capsules, Open Chemistry. 2010; 8(4).
Morshedy S, Omran G, Abduuatef OA, Omar WM. Talaat. Validated spectrofluorimetric method for determination of mirabegron by utilizing its quenching effect on acetoxymercuric fluorescein reagent. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2020;239:118509.
Mongay C, Cerda V. A britton robinson buffer of known ionic strength. Ann. Chim. 1974;64:409-412.
Colovs G, Haro M, Frewer H. Reactions os 2-, 7—Bis(Acetoxymercuri)-fluorescein with certain complexing anions, Talanta. 1970;17:273-278.
Gomez-Hens A, Valchrcel M. Spectrofluorimetric determination anions: A review, Analyst. 1982;107(1274):465-494.
William R, Paul B. Fluorescence quenching studies: analysis of nonlinear Stern-Volmer data, Methods Enzymol. 1992;210:448–463.
Carmody WR. Demonstrating Job’s method with colorimeter or spectrophotometer, J. Chem. Educ. 1964;41(11):615.
Wieslaw M, Tadeusz L. The effect of temperature on the fluorescence quenching of perylene by tetrachloromethane in mixtures with cyclohexane and benzene, Z. Naturforsch. 1992;47a:533–535
Atkins PW, Paula JD, Keeler J. Atkins physical chemistry, Oxford University Press, Oxford; 2018.
ICH. Q2 (R1), Validation of analytical procedures: Text and methodology, international conference on harmonization, Geneva; 2005.
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