International Research Journal of Pure and Applied Chemistry

Capillary  electrophoresis  (CE)  has been developed  as  one  of  the  powerful  separation  techniques  for  the analysis of inorganic and organic ions in complex matrices in the recent past. In aqueous samples, most of the inorganic ions are having weak absorption profiles in the UV-Vis region of the spectrum. These low absorption profile ionic species are commonly detected by indirect UV absorbance with the addition of an absorbing co-ion (chromophore) into the electrolyte. The inorganic cations oftenly require an additional complexing agent to selectively alter their similar mobilities and proper separation. The indirect detection at 214 nm was performed with α-hydroxyisobutyric acid (α-HIBA)-4-aminopyridine background electrolyte (BGE) that has a characteristic absorbance at 214 nm. The BGE was applied for the separation, analysis, and validation of metal ions in water samples. The metal ions such as K⁺, Na+, Ca²⁺, Mg²⁺, Mn²⁺, Fe²⁺, Cd²⁺, Pb²⁺, Ni²⁺ and Zn²⁺ were separated successfully in this study. Also, the effect of electrolyte pH, applied voltage and injection time for the separation of cations was investigated.