Polymer-insertion Effect on Urea Conversion Kinetics via Enzymes Immobilized on Magnetic Microparticles

Jin-Won Park *

Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology, 232 Gongneung-Ro, Nowon-Gu, Seoul, 01811, Republic of Korea.

*Author to whom correspondence should be addressed.


Abstract

Urease was covalently immobilized on the magnetic particles without and with polymer between the urease and the particle. The polymer insertion enhanced the conversion from urea to bicarbonate. The ureases were immobilized on the magnetic microparticles through covalent bonds. 150 μL of stock solution of 3 μm diameter particles was washed three times with 20 mM MES rubbing solution at pH 6.0. The results of this study seem to be useful in designing a reactor for recycling wastewater generated in the semiconductor process.

Keywords: Urea, enzyme immobilization, magnetic microparticles, polymer-insertion, conversion kinetics


How to Cite

Park , Jin-Won. 2023. “Polymer-Insertion Effect on Urea Conversion Kinetics via Enzymes Immobilized on Magnetic Microparticles”. International Research Journal of Pure and Applied Chemistry 24 (5):71-74. https://doi.org/10.9734/irjpac/2023/v24i5827.

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References

Zhang XB, Yang YY, Ngo HH, Guo WS, Wen HT, Wang XA, Zhang JQ, Long TW. A critical review on challenges and trend of ultrapure water production process. Sci. Total Environ. 2021;785:147254.

Choi J, Chung J. Evaluation of urea removal by persulfate with UV irradiation in an ultrapure water production system. Water Res. 2019;158:411-416.

Rydzewski JH, Carr G. Advanced organics oxidation - Removing urea from high-purity water. Ultrapure Water. 2003;20:20-26.

Godec R. Measurement and Removal of Trace Levels of Urea. Ultrapure Water. 2003;20:27-33.

Coulter B, Sundstrom G, Hall C, Doung, S. An Advanced Oxidation Process Update for Removal of Low Organic Levels, Ultrapure Water Micro, Phoenix; 2014.

Joko I, Miwa S. Treatment Method for Water Containing Nitrogen Compounds US Pat. 1997;6132627A.

Karplus PA, Pearson MA, Hausinger RP. 70 years of Crystalline Urease: What Have We Learned? Acc. Chem. Res. 1997;30: 330-337.

Park Y, Kwon J, Jung S, Cho H, Shin G, Cho G, Park J-W, Lee K-H. Urea Conversion via Enzymes Immobilized on Magnetic Microparticles. Korean Chem. Eng. Res. 2023;61:407-411.

Kang MJ, Park J-W. Amplification of Urea Detection Based on pH-sensitive Liposomes. Electron. J. Biotechnol. 2021; 52:30-34.

Andrich L, Esti M, Moresi M. Urea Degradation Kinetics in Model Wine Solutions by Acid Urease Immobilised Onto Chitosan-derivative Beads of Different Sizes Enzyme Microb. Technol. 2010;46:397-405.

Lasisi AA, Akinremi OO. Kinetics and Thermodynamics of Urea Hydrolysis in the Presence of Urease and Nitrification Inhibitors Can. J. Soi Sci. 2021;101:192-202.