Anti-Diabetic Activities of the Extracts from Euphorbia hirta L. (Euphorbiaceae) Specie found in Burkina Faso Using α-Glucosidase Inhibitor
Yssouf Karanga *
Laboratoire de Chimie Analytique Environnementale et Bio-Organique (LCAEBiO), Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina Faso and Laboratoire de Chimie Analytique, de Physique Spatiale et Energétique (L@CAPSE), Université Norbert ZONGO, Avce Maurice Yameogo, Koudougou BP 376, Burkina Faso.
Têeda Hamidou Ganamé
Laboratoire de Chimie Analytique Environnementale et Bio-Organique (LCAEBiO), Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina Faso.
Wende-Konté Hazael Conania Nikiema
Laboratoire de Chimie Analytique Environnementale et Bio-Organique (LCAEBiO), Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina Faso.
Issa Tapsoba
Laboratoire de Chimie Analytique Environnementale et Bio-Organique (LCAEBiO), Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Euphorbia hirta L. is an herbaceous plant used in traditional medicine to treat several diseases. In the present work, different fractions of Euphorbia hirta L. hydroalcoholic extract were investigated for their anti-diabetic properties on α-glucosidase’s inhibition activities. Chemical screening realized following Folin-Ciocalteu and AlCl3 methods showed that ethyl acetate (AcOET) and butanol (BuOH) extracts contain 432.687 ± 1.832 µg and 332.433 ± 1.255 µg GAE/mg of extract for phenolic compounds. The same extracts contained high levels of total flavonoids estimated at 417.850 ± 15.941 µg and 227.640 ± 2.657 μg QE/mg of extract respectively. The anti-diabetic activities revealed that AcOET and BuOH have a very significant inhibitory potential on the enzymatic activity of α-glucosidase with an IC50 values of 1.295 ± 0.035 μg/mL and 2.188 ± 0.204 μg/mL respectively compared to acarbose (IC50 = 214.825 ± 3.816 μg/mL).
Keywords: Euphorbia hirta L, α-glucosidase, diabetes, flavonoids, inhibition