Aims: In the present study our aim is to develop and validate a reverse phase HPLC method for both determination and quantification of cochicine in different parts of Gloriosa superba. The developed HPLC method is validated for parameters as mentioned in ICH guidelines.
Place and Duration of Study: Study was undertaken in Department of Forest Products, University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India and in the period between June 2015 and July 2016. The plant material used for testing of developed and validated method was collected in October, 2015 and is identified in the Herbarium section of the above said Department with reference number 13900.
Methodology: The system used is of Waters binary HPLC unit with Waters HPLC pump 515, dual λ absorbance detector 2487 and Empower II software. Standard of colchicine was purchased from Sigma Aldrich, (USA) and was used for HPLC method development and validation. The developed HPLC method was validated for parameters as mentioned in ICH guidelines.
Results: The analytical column, Sunfire C18 (4.6×250mm, 5µm) was operated at ambient temperature. Isocratic elution with A acetonitrile and B 3% glacial acetic acid was at a flow rate of 1 ml/min. UV detection was done at 245nm and run time was given ten minutes for standards and fifteen minutes for samples. LOD and and LOQ of the developed method was found as 0.002 µg/ml and 0.011 µg/ml respectively.
Conclusion: Method was found to be satisfactory in terms of high accuracy, precision and robustness. The method was successfully applied to the extracts made of different plant parts of Gloriosa superba.
Essential oils derived from aromatic plants have exhibited biological properties and can be used to prevent and treat human diseases. The goal of this work was to investigate the antibacterial and antifungal potential of the essential oils extracted from R. officinalis and P. citrosum against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis) and Gram-negative bacteria (Pseudomonus aeruginosa, Escherichia coli) and fungus (Candida albicans). The antimicrobial activities of the R. officinalis and P. citrosum essential oils were carried out using the Disk diffusion method. The results indicated that the essential oils from P. citrosum had antimicrobial activity against Bacillus subtilis and Escherichia coli and Candida albicans at a low concentration of 0.5 % v/v and that the activity was concentration dependent. Essential oil from R. officinalis on the other hand showed effective antimicrobial activity against Escherichia coli, Staphylococcus aureus and Pseudomonus aeruginosa and Candida albicans. P. citrosum was found to be more effective than Nitrofuractoin and Gentamicin drugs against Staphylococcus aureus at a higher concentration of 6% v/v. R. officinalis oil extracts also demonstrated similar trends and were comparable to the positive controls against the tested microbes. It was therefore concluded that R. officinalis and P. citrosum plant extracts were effective against the tested antimicrobial agents and have potential to be used against the tested microbes.
Structure of nine quinazoline derivatives were optimized using density functional theory method in order to probe into the bioactive conformations of the compound. The obtained descriptors which described the anti-neuroepithelioma activity of the compounds were selected and used to develop a model using partial least square method. The developed model replicated the experimental IC50 indicative of the predicting power of the model. In addition, ligand-receptor interactions are reported and 2-((E)-2-(4-Bromo-phenyl)-vinyl)-3H-quinazolin-4-one (A4) showed the greatest affinity to bind on the active site of human neuroepithelioma cell line.
A heterogeneous catalyst, rice husk supported calcium chloride dihydrate (RiH-CaCl2.2H2O), has been developed and characterized using energy dispersive spectroscopy (EDS), fourier transform infra-red (FT-IR) spectroscopy and scanning electron microscopy (SEM). RiH-CaCl2.2H2O offers simple, efficient and economical solid support synthetic protocol for the synthesis of formamides under solventless condition at room temperature to afford the formamide derivatives. The ability of RiH-CaCl2.2H2O to enhance the reaction rate is described in terms of preorganizing effect. This method provides green approach for N-formylation and easy isolation process. The method is superior over the existing methods as it utilizes methanolic acid in lesser amount and works well at room temperature.
The soxhlet extraction of Moringa seed oil was used to determine the proximate and physicochemical screening. The parameters obtained for the proximate screening were 7.64% moisture content, 4.05% ash content, 29.65% crude fat, 34.92% crude protein and 52.30% carbohydrate while the values obtained for the physico-chemical screening were 62.45% for Iodide, 1.1% for specific gravity, 9.84 for free fatty acid, 162.84% for saponification value, 4.10% for peroxide value, 1.46% for refractive index, 10.50% for viscosity and 5.95% for acid value. The results showed that Moringa oleifera seeds and seed oil could be employed for edible and commercial purposes.