Simultaneous Determination of Camylofin Dihydrochloride and Paracetamol Using Differential Pulse Voltammetry in Micellar Media
Emad Mohamed Hussien
Department of Pharmaceutical Chemistry, National Organization for Drug Control and Research (NODCAR), 9 Abou-Hazem str., Giza, Egypt.
Israa Abd El-Ghafar Mohammed *
Department of Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Cairo, 11754, Egypt.
Sawsan Abdel-Moneim Abd El-Razeq
Department of Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Cairo, 11754, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Simple and sensitive differential pulse voltammetric (DPV) method has been developed for simultaneous determination of camylofin-2HCl and paracetamol. The electrochemical oxidation of camylofin was studied at carbon paste electrode using cyclic and differential pulse voltammetry. A reproducible and reliable oxidation current peak was obtained at pH 7. The oxidation current was enhanced in presence of sodium dodecylsulphate as an erosion reagent. Under optimal conditions the oxidation current was proportional to camylofin concentration in the range from 5.0 to 100.0 µM. Besides, the method was linear for paracetamol which is an active ingredient in the concentration range from 5.0 to 1000.0 µM. The proposed DPV method was selective for simultaneous determination of the two drugs with mean recovery of 99.58±2.13% and 99.44±0.57% for camylofin-2HCl and paracetamol, respectively. The method was validated according to ICH guidelines and successfully applied for the determination of the two drugs in combined pharmaceutical formulations.
Keywords: Camylofin, electro-oxidation, carbon paste electrode, differential pulse voltammetry, micellar media.