Identification of Five Acylated Anthocyanins and Determination of Antioxidant Contents of Total Extracts of a Purple-Fleshed Ipomoea batatas L. Variety Grown in Burkina Faso
Dominique Saga Kaboré
Laboratoire de Chimie Organique et de Physique Appliquées, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Adama Héma *
Laboratoire de Chimie Organique et de Physique Appliquées, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Elie Kabré
Laboratoire National de Santé Publique 09 BP 24 Ouagadougou 09, Burkina Faso.
Raoul Bazié
Laboratoire National de Santé Publique 09 BP 24 Ouagadougou 09, Burkina Faso.
Abdoul Karim Sakira
Laboratoire de Chimie Analytique et de Toxicologie, UFR/ Sciences de la Santé, Université Joseph Ki-Zerbo, Burkina Faso.
Moumouni Koala
Laboratoire de Chimie Organique et de Physique Appliquées, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso and Institut de Recherche en Sciences de la Santé (IRSS/CNRST) 03 BP 7192 Ouagadougou 03, Burkina Faso.
Paul-André Koussao Somé
CNRST/INERA, Département Productions Végétales, 03 BP 7047 Ouagadougou 03 Burkina Faso.
Eloi Palé
Laboratoire de Chimie Organique et de Physique Appliquées, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Touridomon Issa Somé
Laboratoire de Chimie Analytique et de Toxicologie, UFR/ Sciences de la Santé, Université Joseph Ki-Zerbo, Burkina Faso.
Pierre Duez
Service de Chimie Thérapeutique et de Pharmacognosie, Université de Mons, 25 Chemin du Champ de Mars, 7000 Mons, Belgique.
*Author to whom correspondence should be addressed.
Abstract
Anthocyanins are bioactive compounds, which thanks to their anti-free radical properties, can protect the human body against oxidative stress. The latter can cause many diseases, such as cancer, aging. The extract of the green cap purple variety of the sweet potato (Ipomoea batatas L.) is active against the radical ABTS. Its antioxidant content is estimated at 0.183 mg E TEAC/ g fresh material. The characterization of anthocyanins was performed by high performance liquid chromatography-mass spectrometry-UV (HPLC-MS-UV) and high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) analyses. These analyses allowed the identification of five anthocyanic compounds. These are: cyanidin 3-(6''-caffeoyl- 6'''-p-hydroxybenzoyl sophoroside) -5-glucoside; cyanidin 3-(6''-feruloyl 6'''-caffeoyl sophoroside) -5-glucoside; peonidin 3-(6'''-caffeoyl sophoroside)-5-glucoside; peonidin 3-(6''-caffeoyl-6'''-p-hydroxybenzoyl sophoroside) -5-glucoside; and peonidin 3-(6''-feruloyl -6'''-caffeoyl sophoroside)-5-glucoside.
Keywords: Anthocyanins, high performance liquid chromatography, mass spectrometry