Comparative Analysis of Titrimetric Methods for Quantifying Acetylsalicylic Acid in Aspirin Tablets
Maida Šljivić Husejnović *
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Alija Uzunović
Agency for Medicinal Products and Medical Devices of Bosnia and Herzegovina, Maršala Tita 9, 71000 Sarajevo, Bosnia and Herzegovina.
Svjetlana Babić
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Slavko Kovač
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Azra Suljić
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Merima Ibišević
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Amra Džambić
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Aida Smajlagić
Faculty of Natural Sciences, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Ermina Cilović Kozarević
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Enida Karić
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
*Author to whom correspondence should be addressed.
Abstract
This study compares two titrimetric methods for quantifying acetylsalicylic acid (ASA) in aspirin tablets stored under different environmental conditions. ASA stability can be influenced by factors such as temperature, humidity, and light exposure. The two titrimetric methods used are acid-base titration with hydrochloric acid (HCl) and sodium hydroxide (NaOH). Aspirin tablets were stored for 30 days under controlled conditions simulating varying environmental factors, and both methods were evaluated for accuracy, precision, and reliability. The results show a strong correlation between the two methods, with a Pearson correlation coefficient of 0.937 and a high Intraclass Correlation Coefficient (ICC), indicating consistency and reliability. However, the paired t-test revealed a statistically significant difference (r = 0.937, p = 0.001) between the methods, suggesting small but meaningful discrepancies in their results. The Bland-Altman analysis demonstrated that Method I consistently provided higher values than Method II, while the linear regression analysis indicated that Method II slightly underestimates values compared to Method I. Overall, both methods were found to be highly reliable and interchangeable within certain limits, but the small systematic differences between them should be considered when interpreting results. This study provides valuable insights into the performance of titrimetric methods for ASA quantification, contributing to the optimization of pharmaceutical analysis techniques.
Keywords: Pharmaceutical analysis, acetylsalicylic acid, titrimetric methods, aspirin tablets